Composite

Part:BBa_K4378008

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)


MHETase expression cassette

This is an expression cassette for IPTG inducible overexpression of MHETase (BBa_K4378002) in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1421
    Illegal BglII site found at 1736
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 420
    Illegal AgeI site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None